Bioluminescent Reporter Screens: Illuminating Novel Regulators and Dynamics of Signaling Pathways
Prof. David Piwnica-Worms. Department of Cancer Systems Imaging Deputy Division Head, Research Affairs, Division of Diagnostic Imaging Gerald Dewey Dodd, Jr., Endowed Distinguished Chair in Diagnostic Imaging The University of Texas M.D. Anderson Cancer C18 November 2016 4:00pmSmall Lecture Theatre, Cavendish LaboratoryBioluminescent imaging (BLI) is a powerful noninvasive tool that has dramatically accelerated the in vivo interrogation of cancer systems and longitudinal analysis of mouse models of cancer over the past decade. Various luciferase enzymes have been genetically engineered into cells and mouse models of cancer, which permit investigation of cellular and molecular events associated with oncogenic transcription, posttranslational processing, protein–protein interactions, transformation, and oncogene addiction in live cells and animals. Luciferase-coupled reporters ultimately serve as a noninvasive, repetitive, longitudinal, and physiologic means by which cancer systems and therapeutic responses can be investigated accurately within the context of a living animal.